Next generation sequencing technology has made it easier than ever to diagnose plant diseases quickly. “It’s really exciting to see how sequencing technologies have evolved and how this new technology enables entire genomes to be sequenced in such a short amount of time,” said Yazmín Rivera, plant pathologist with the Department of Agriculture’s Plant Protection and Quarantine Program. who recently published a research paper on the effectiveness of protocols from Oxford Nanopore Technologies.
“We wanted to provide an unbiased assessment of the technology and protocols available for long-read sequencing,” said Rivera. Together with other plant pathologists, Rivera used the company’s protocols to create RNA and DNA libraries from virus-infected plant material and from a phytopathogenic bacterium, respectively. After an hour of data sequencing, the scientists had enough data to assemble small genomes.
“Diagnostics will appreciate an objective review of this technology,” said Rivera. Rivera and her colleagues published their findings in Plant Health Progress and presented a head-to-head comparison of protocols that can help readers determine which library prep kit is best for their needs.
The ability to quickly obtain the entire genome of an organism has a major impact on the field of plant pathology. “As more information becomes available, emerging and difficult-to-identify pathogens can be identified,” explains Rivera. For more information, see “Comparison of Nanopore Sequencing Protocols and Real-Time Analysis for Phytopathogen Diagnostics”. published in the March issue of Plant Health Progress.
Targeting DNA Sequencing for the Study of Plant Biodiversity
More information:
Tommy Phannareth et al., Comparison of Nanopore Sequencing Protocols and Real-Time Analysis for Phytopathogen Diagnostics, Progress in Plant Health (2020). DOI: 10.1094 / PHP-02-20-0013-RS
Provided by the American Phytopathological Society
Quote: New technology enables the rapid sequencing of entire genomes of plant pathogens (2021, May 14), accessed on May 14, 2021 from https://phys.org/news/2021-05-technology-enables-rapid-sequencing-entire. html
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